Thermal profiles and primer used for real-time PCR quantification of different functional genes.
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PCR reaction mixtures with a final volume of 25 µl consisted of Power SybrGreen Master Mix (12.5 µl), BSA (15 µg), template (2 µl) as well as primer and DMSO in a final concentration as referred in the table.aTouchdown: −1°C cycle−1.
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2015-12-02



