Data underlying the publication: Induced intra- and intermolecular template switching as a therapeutic mechanism against RNA viruses

The research object was the characterization of two enterovirus RNA-dependent RNA polymerases using multiplexed magnetic tweezers, to investigate snap-back synthesis and recombination. Presented here are <i>in vitro</i> measurements of the bead position during RNA replication. Bead position measurements were taken for 2 hours a constant pulling force of 25 pN at 24C at a camera acquisition rate of 50 Hz. Traces were cropped from the start until the end of the change in z-position, which was converted to nucleotides.<br><br>

本研究以两种肠道病毒RNA依赖RNA聚合酶（RNA-dependent RNA polymerase）为研究对象，采用多重磁镊技术对其进行表征，旨在探究回弹合成（snap-back synthesis）与重组过程。

本文展示的是RNA复制过程中磁珠位置的体外（in vitro）检测数据。磁珠位置检测时长为2小时，实验过程中施加25皮牛（pN）的恒定拉力，环境温度为24摄氏度，相机采集速率为50赫兹（Hz）。数据轨迹从z轴位置变化的起始点截取至结束点，该位置变化已被换算为对应核苷酸数量。