T505A variant in ETV5 promotes proliferation of precursor B cells in a mouse model of acute lymphoblastic leukemia

The goal of this project was to determine the effect of forced expression of ETS-translocation-variant-5 (ETV5) wild-type, R392P, V444I, and T505A variants on gene expression in a cultured pre-B cell line SeptMBr. Total RNA was prepared from SeptMBr interleukin-7-dependent pre-B cells transduced with control retroviral vector (MIEG3), or retroviral vectors encoding wild-type, R392P, V444I, or T505A ETV5 variants (3 biological replicates of each). This is a total of 15 biosamples. For each biosample, polyA enriched libraries were prepared and sequenced using the Illumina NovaSeq PE 100-25M reads by Genome Quebec. Each biosample consists of a BAM file containing aligned paired end reads (Mm10, GRCm38).