Analysis of B cell neighborhoods via single cell spatial transcriptomics in head and neck cancer patients. Analysis of B cell neighborhoods via single cell spatial transcriptomics in head and neck cancer patients

Head and neck squamous cell carcinoma tissue microarrays were stained via CD20 single plex IHC and then FOVs were selected for B cell neighborhoods (including tertiary lymphoid structures) in confirmed tumor areas via H&E evaluation by a pathologist. Overall design: In this study, 1000 plex CoSMX via Nanostring was performed on 11 patients with multiple FOVs for B cell neighborhoods