RNA-seq of human fibroblast cell cultures treated with resveratrol against DMSO treated controls, with and without siRNA treatment targeting SIRT1

Resveratrol (RSV) is a small compound with many reported beneficial effects, including increased expression of genes involved in inborn errors of metabolism. We wanted to investigate the effects of RSV on gene regulation on a global scale in order to detect new target genes for RSV mediated up-regulation with respect to disease treatment. Because fibroblasts are widely used diagnostically and reflect many metabolically important tissues well, we chose to investigate normal fibroblasts treated with RSV. We performed this with and without knock down of sirtuin 1 (SIRT1) since SIRT1 has been reported as a key mediator of RSV effects. We performed Next Generation Sequencing of RNA on 2 normal fibroblast cell line with and without the respective treatments.