Dilution series 40nm SA-AgNP on CD8-bio

Determine optimal concentration for usage of 40 nm SA-AgNP in mass cytometry Done on PBMC with CD8-bio as primary antibody Repetition of an older experiment for publication Conclusion: Best signal with conc 1:25, but problems in all other channels (incomplete ionization, due to high metal content? (1:25 - 1:100). Best signal without this issue: 1:200. No sufficient resolution at titers above 1:400 Notes: see Fig 1 D-E Comparison to direct detection by CD8-168Er (serves also as no primary ab control)