chlange/streptococcus_thermophilus_fermentation_timegate

---
license: cc-by-4.0
tags:
- raman-spectroscopy
- time-gated-raman
- biotechnology
- streptococcus-thermophilus
- fermentation
- batch-cultivation
- process-analytical-technology
pretty_name: Time-Gated Raman Spectra of Streptococcus thermophilus Fermentations
language:
- en
size_categories:
- n<1K
---

# Dataset Card for Time-Gated Raman Spectra from Fermentations of Streptococcus thermophilus

## Dataset Summary
This dataset contains offline Raman spectra collected during batch cultivations of ***Streptococcus thermophilus***. Unlike conventional continuous-wave Raman, these measurements were captured using **Time-Gated Raman Spectroscopy**, which allows for the temporal separation of the Raman signal from background fluorescence.

The dataset includes two distinct fermentation runs conducted in shake flasks over a 24-hour period, paired with reference analytical measurements (HPLC and spectrophotometry).

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## Dataset Structure

### Data Points
Each entry consists of a Raman spectrum (intensity vs. Raman shift) paired with the following quantitative annotations:

* **Lactose [g/L]:** Primary carbon source.
* **Galactose [g/L]:** Metabolic byproduct.
* **Lactate [g/L]:** Primary fermentation product.
* **OD600:** Optical density at 600 nm (biomass concentration).

### Preprocessing
* **Status:** Raw.
* **Note:** The spectra are provided in their raw format. No computational fluorescence rejection, baseline correction, or smoothing has been applied. The time-gating hardware provides the initial fluorescence suppression.

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## Technical Specifications

### Cultivation Parameters
* **Organism:** *Streptococcus thermophilus*
* **Cultivation Mode:** Batch
* **Vessel:** Shake flask
* **Duration:** ~24 hours

### Measurement Setup
* **Spectrometer:** Timegate PicoRaman (First Generation)
* **Technology:** Time-Gated Raman Spectroscopy (pulsed laser)
* **Sampling:** Offline samples taken throughout the fermentation.
* **Reference Analytics:** * Metabolites measured via High-Performance Liquid Chromatography (HPLC).
* Biomass measured via UV-Vis Spectrophotometry ($OD_{600}$).

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## Dataset Sources

- **Repository:** [https://huggingface.co/datasets/chlange/streptococcus_thermophilus_fermentation_timegate](https://huggingface.co/datasets/chlange/streptococcus_thermophilus_fermentation_timegate)
- **Paper:** [Link to paper if available]

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