chlange/streptococcus_thermophilus_fermentation_timegate

--- license: cc-by-4.0 tags: - raman-spectroscopy - time-gated-raman - biotechnology - streptococcus-thermophilus - fermentation - batch-cultivation - process-analytical-technology pretty_name: Time-Gated Raman Spectra of Streptococcus thermophilus Fermentations language: - en size_categories: - n<1K --- # Dataset Card for Time-Gated Raman Spectra from Fermentations of Streptococcus thermophilus ## Dataset Summary This dataset contains offline Raman spectra collected during batch cultivations of ***Streptococcus thermophilus***. Unlike conventional continuous-wave Raman, these measurements were captured using **Time-Gated Raman Spectroscopy**, which allows for the temporal separation of the Raman signal from background fluorescence. The dataset includes two distinct fermentation runs conducted in shake flasks over a 24-hour period, paired with reference analytical measurements (HPLC and spectrophotometry). --- ## Dataset Structure ### Data Points Each entry consists of a Raman spectrum (intensity vs. Raman shift) paired with the following quantitative annotations: * **Lactose [g/L]:** Primary carbon source. * **Galactose [g/L]:** Metabolic byproduct. * **Lactate [g/L]:** Primary fermentation product. * **OD600:** Optical density at 600 nm (biomass concentration). ### Preprocessing * **Status:** Raw. * **Note:** The spectra are provided in their raw format. No computational fluorescence rejection, baseline correction, or smoothing has been applied. The time-gating hardware provides the initial fluorescence suppression. --- ## Technical Specifications ### Cultivation Parameters * **Organism:** *Streptococcus thermophilus* * **Cultivation Mode:** Batch * **Vessel:** Shake flask * **Duration:** ~24 hours ### Measurement Setup * **Spectrometer:** Timegate PicoRaman (First Generation) * **Technology:** Time-Gated Raman Spectroscopy (pulsed laser) * **Sampling:** Offline samples taken throughout the fermentation. * **Reference Analytics:** * Metabolites measured via High-Performance Liquid Chromatography (HPLC). * Biomass measured via UV-Vis Spectrophotometry ($OD_{600}$). --- ## Dataset Sources - **Repository:** [https://huggingface.co/datasets/chlange/streptococcus_thermophilus_fermentation_timegate](https://huggingface.co/datasets/chlange/streptococcus_thermophilus_fermentation_timegate) - **Paper:** [Link to paper if available] ---