Supporting information_In situ rolling circle amplification Förster resonance energy transfer (RCA-FRET) for washing-free real-time single protein imaging

Chemical structures of the lanthanide complexes used for TG imaging; TG and CW images of in situ RCA in HaCaT cells detected with three different organic dyes and three different lanthanide complexes; overlay images of DIC and PL for CW and TG RCA-FRET; determination of signal-to-background ratios; in situ RCA imaging with single Tb and Cy3.5 probes and without probes; Tb and Cy3.5 PL spectra in different aqueous solutions with and without FA; images of RCP-probe hybridization over time in Tb and Cy3.5 detection channels.