Next Generation Sequencing Analysis of human hematopoietic stem/progenitor cells (HSPCs) differentiated from pluripotent stem cells
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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Purpose: global gene expression profiling of H1 and iPSC derived HSPCs.Methods: SMART-seq2 amplified polyA RNA from differentiation day 8 CD31+CD34+CD43+ and CD31+CD34+CD43- cells, and day 5 CD31+CD34+ cells cultured in medium with or without insulin supplementResults: Modulation of key cell signaling pathways lead to highly efficient HSPC differentiation from human pluripotent stem cells.Conclusions: Highly efficient HSPC differentiation from human pluripotent stem cells in a monolayer, chemically defined culture system.
提供机构：
Tsinghua University
创建时间：
2022-02-20
