Dataset of the acute toxicity of mixtures of a copper dihydroxide-based nanopesticide or copper chloride with three commercial pesticide formulations on Daphnia magna, including recovery of active ingredients and characterization of nanoparticles

This dataset includes data of the acute toxicity of mixtures of a copper dihydroxide-based nanopesticide or copper chloride with three commercial pesticide formulations on Daphnia magna, including recovery of active ingredients and characterization of nanoparticles. The tested products are Funguran progress (FP), acetamiprid (ACE), prochloraz (PRO), glyphosate (GLY) and copper chloride (CuCl2). The file named "Immobilization" includes data of the miniaturized acute daphnia toxicity test from all tested single substances and their mixtures with either Funguran progress as the copper hydroxide-based nanopesticide or copper chloride as the ion control. All results were taken after 48 h of exposure, where "n" denotes the total no. of daphnids teste, "k, immobile" the no. of immobilized daphnids. Tested concentrations "x" of the active ingredients were log-transformed after addition of a small number, allowing for inclusion of the negative control wich would have otherwise included 0 values. Also included are EC50 values calculated from dose response cruves that were generated with generalized linear models. The dose-response curves were used to calculate the toxic units at different ECx values. These calculations were also included. The file named "Chemical Analysis" includes recovery data of the active ingredients from the test solutions, with which the miniaturized acute daphnia toxicity test was conducted. Measured concentrations are shown for only the active ingredients in the product formulations, or for copper in case of FP and CuCl2. Copper concentrations were measured with atomic adsorption spectroscopy, GLY, ACE and PRO were measured with HPLC connected to a diode array detector. "Recovery" refers to the percentage of recovers substance compared to the originally added concentration. The file named "Nanoparticle Characterization" includes data on measurement of nanoparticle size and surface charge of the copper dihydroxide nanoparticles in different media and in mixtures with ACE, PRO and GLY. Nanoparticle sizes were measured with dynamic light scattering (DLS) and surface charge changes (zeta potential) were determined with electrophoretic light scattering (ELS). The file also includes data on an experimente quantifying the dissolution of copper ions from the copper dihydroxide nanoparticles. Copper was quantified at two timepoints and before and after ultracentrifugation to include either all copper in the sample or only the dissolved ion fraction for the copper dihydroxide nanopesticide itself and its mixtures with ACE, PRO and GLY