Data_Sheet_1_Structural identification of pyridinopyrone compounds with anti-neuroinflammatory activity from streptomyces sulphureus DSM 40104.PDF

This study investigated the chemical composition and biosynthesis pathway of compounds produced by Streptomyces sulphureus DSM 40104. With the guild of molecular networking analysis, we isolated and identified six uncommon structural characteristics of compounds, including four newly discovered pyridinopyrones. Based on genomic analysis, we proposed a possible hybrid NRPS-PKS biosynthesis pathway for pyridinopyrones. Notably, this pathway starts with the use of nicotinic acid as the starting unit, which is a unique feature. Compounds 1–3 exhibited moderate anti-neuroinflammatory activity against LPS-induced BV-2 cell inflammation. Our study demonstrates the diversity of polyene pyrone compounds regarding their chemical structure and bioactivity while providing new insights into their biosynthesis pathway. These findings may lead to the development of new treatments for inflammation-related diseases.

本研究对Streptomyces sulphureus DSM 40104产生的化合物的化学组成和生物合成途径进行了探究。借助分子网络分析技术，我们成功分离并鉴定出六种不常见的化合物结构特征，其中包括四种新发现的吡啶并吡喃类化合物。基于基因组分析，我们提出了一个可能的混合NRPS-PKS生物合成途径，该途径以烟酸作为起始单元，具有独特性。化合物1-3对LPS诱导的BV-2细胞炎症表现出中等程度的抗神经炎症活性。本研究展示了多烯吡喃类化合物在化学结构和生物活性方面的多样性，并为它们的生物合成途径提供了新的见解。这些发现可能为炎症相关疾病的新疗法开发奠定基础。