Expression data from white and brown adipose tissue (WAT and BAT) of per2-/- and control mice

We found that the circadian protein PER2 interacts with the nuclear receptor PPARgamma to repress its activity. PPARgamma is a master regulator of adipogenesis and lipid metabolism and is very abundant in adipose tissue. We used microarrays to detail the global program of gene expression in adipose tissue lacking the per2 gene. This analysis identified several PPARgamma target genes up-regulated in adipose tissue from per2-/- mice. Per2-/- and per2+/+ male mice (Bae et al., 2001) were housed under 12 hr light/12 hr dark (LD) cycles. Mice 20 weeks old were sacrificed at the same time and adipose tissue (WAT and BAT) was collected for RNA extraction. 3 biological replicates per mouse/tissue.我们发现节律蛋白PER2（circadian protein PER2）可与核受体PPARγ（nuclear receptor PPARγ）相互作用并抑制其活性。PPARγ是脂肪生成与脂质代谢的主控调控因子，在脂肪组织中表达丰度极高。本研究采用基因芯片（microarrays）对缺失per2基因（per2 gene）的脂肪组织的全局基因表达程序进行了详细解析，该分析鉴定出多个在per2⁻/⁻小鼠脂肪组织中上调的PPARγ靶基因。实验所用的per2基因敲除型（per2⁻/⁻）与野生型（per2⁺/⁺）雄性小鼠（Bae等，2001）均饲养于12小时光照/12小时黑暗（LD）光周期环境中。选取20周龄的小鼠于同一时间点处死，采集脂肪组织，包括白色脂肪组织（white adipose tissue, WAT）与棕色脂肪组织（brown adipose tissue, BAT），用于RNA提取。每个小鼠/组织设置3次生物学重复（biological replicates）。