Friedhoff et al. (2024) - Fig 2 FACS data

Fig 2. PEI transfection of SARS-CoV-2 replicon DNA leads to GFP expression that is dependent on DNA linearization but not T7 RNAP and is remdesivir-insensitive. HEK293T cells transduced with the empty retroviral vector LPCX(AB) (A) or with LPCX(AB)-T7RNAP (B) were PEI-transfected with the pSMART-BAC-T7-scv2 replicon or were mock-transfected as a control. BAC DNA was linearized or not with SwaI immediately prior to transfections. Two independent replicon DNA transfections were done for both non-linearized and linearized replicon DNA. For the linearized replicon, an additional transfection was performed in the presence of 100 nM remdesivir (bottom left dot plot). GFP expression was analyzed two days post-transfection by flow cytometry.