five

RNA Flow Cytometry: multiplexing evaluation

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http://flowrepository.org/id/FR-FCM-ZZCE
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The purpose was to evaluate the multiplexing capability of RNA flow cytometry by staining 3 mRNA targets simultaneously. Conclusion: After RNA staining, we were able to confirm positive staining for all three targets: BCR, ABL, and 18s. Cells were sorted and also validated by imaging. Notes: Detection of Low Abundance RNA Molecules in Individual Cells by Flow Cytometry Figure 4A-B Instrument was setup using CST in FACSDiva software.
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2014-05-01
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