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pAmidase6H and p8S1-MM6 plasmid maps

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DataCite Commons2025-04-15 更新2025-05-07 收录
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https://figshare.com/articles/dataset/pAmidase6H_and_p8S1-MM6_plasmid_maps/28800926/1
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pAmidase6H was created for heterologous expression of the amidase gene <i>amiX </i>from <i>Synechococcus elongatus </i>sp. PCC 7942 in an <i>Escherichia coli </i>host strain with a C-terminal hexahistidine tag.<br><br>p8S1-MM6 was designed for inactivation of the Synpcc7942_1548 (<i>amiX</i>) gene locus in <i>Synechococcus elongatus </i>sp. PCC 7942 by insertion of a kanamycin resistance cassette. <br><br>Both plasmids were first described in "A responsive living material prepared by diffusion reveals extracellular enzyme activity of cyanobacteria", DOI: 10.1073/pnas.2424405122
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2025-04-15
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