Termite cultivation project

This project barcoded bacterial and fungal microbial communities in samples related to several termite species. Samples were collected in French Guiana, Papua New Guinea and Cameroon. We sequenced microbial communities living in the guts of the species Anoplotermes banksi, Cephalotermes sp., Cubitermes sp., Microcerotermes sp. and Nasutitermes sp. We also sampled related termite environments associated with each sampled species: soil or wood food substrate and nest material. We also cultivated microorganisms from each sampled environment (except guts), and determined their relevant macroscopic characteristics, and further elucidated their genera and species through Sanger sequencing. The sequencing strategy (barcoding) consisted of a first step of amplification with tagged primers and sequencing by pools. The pool information can be extracted from the list below (tab separated). sample-id sample-id f-barcode-sequence r-barcode-sequence geographical-location replicate sample-origin seq-type species worker-type pool pool_b #q2:types #q2:types categorical categorical categorical numeric categorical categorical categorical categorical numeric categorical CAM_Cephalo_GUTS_1 Cep_Gu_1 TCCACTTGTGTGCCAGCMGCCGCGGTAA TATTCAACCGGACTACHVGGGTWTCTAAT Cameroon 1 Gut 16s Cephalotermes sp. Normal 6 6 CAM_Cephalo_GUTS_2 Cep_Gu_2 TAAGAATGTGTGCCAGCMGCCGCGGTAA TTAACATCCGGACTACHVGGGTWTCTAAT Cameroon 2 Gut 16s Cephalotermes sp. Normal 6 6 CAM_Cephalo_GUTS_3 Cep_Gu_3 TGCGTGTGTGCCAGCMGCCGCGGTAA TAATAGGCCGGACTACHVGGGTWTCTAAT Cameroon 3 Gut 16s Cephalotermes sp. Normal 6 6 CAM_Cephalo_Nest_19071 Cep_Ne_1 TGGACTTGTGTGCCAGCMGCCGCGGTAA TAACAATCCGGACTACHVGGGTWTCTAAT Cameroon 1 Nest 16s Cephalotermes sp. Not applicable 5 5 CAM_Cephalo_Nest_19072 Cep_Ne_2 TCGTTGTGTGCCAGCMGCCGCGGTAA TCCGTCTCCGGACTACHVGGGTWTCTAAT Cameroon 2 Nest 16s Cephalotermes sp. Not applicable 1 1 CAM_Cephalo_Nest_19073 Cep_Ne_3 TGCACCTGTGTGCCAGCMGCCGCGGTAA TTAGACACCGGACTACHVGGGTWTCTAAT Cameroon 3 Nest 16s Cephalotermes sp. Not applicable 5 5 CAM_Cephalo_WF_190719_1 Cep_Wf_1 TAATCGTGTGTGCCAGCMGCCGCGGTAA TCACATACCGGACTACHVGGGTWTCTAAT Cameroon 1 Wood food substrate 16s Cephalotermes sp. Not applicable 5 5 CAM_Cephalo_WF_190719_2 Cep_Wf_2 TCAACTCGTGTGCCAGCMGCCGCGGTAA TAGCCGTCCGGACTACHVGGGTWTCTAAT Cameroon 2 Wood food substrate 16s Cephalotermes sp. Not applicable 5 5 CAM_Cephalo_WF_190719_3 Cep_Wf_3 TCCGATTGTGTGCCAGCMGCCGCGGTAA TAATTCGCCGGACTACHVGGGTWTCTAAT Cameroon 3 Wood food substrate 16s Cephalotermes sp. Not applicable 1 1 CAM_Cubi_GUTS Cubi_Gu TGAGGCTGTGTGCCAGCMGCCGCGGTAA TATCTGACCGGACTACHVGGGTWTCTAAT Cameroon 1 Gut 16s Cubitermes sp. Normal 6 6 CAM_Cubi_nest_190430 Cubi_Ne TCGCTTCGTGTGCCAGCMGCCGCGGTAA TTCCAGGCCGGACTACHVGGGTWTCTAAT Cameroon 1 Nest 16s Cubitermes sp. Not applicable 5 5 CAM_SF_Cubitermes Cub_Sf TGCATCGTGTGCCAGCMGCCGCGGTAA TGCAAGCCGGACTACHVGGGTWTCTAAT Cameroon 1 Soil food substrate 16s Cubitermes sp. Not applicable 1 1 FG_Anoplo_GUTS_1 Anp_Gu_1 TGCGCGAGTGTGCCAGCMGCCGCGGTAA TGTGCGTCCGGACTACHVGGGTWTCTAAT French Guiana 1 Gut 16s Anoplotermes banksi Normal 6 6 FG_Anoplo_GUTS_2 Anp_Gu_2 TTGCGAGTGTGCCAGCMGCCGCGGTAA TGAATCGCCGGACTACHVGGGTWTCTAAT French Guiana 2 Gut 16s Anoplotermes banksi Normal 5 5 FG_Anoplo_GUTS_3 Anp_Gu_3 TATTAATGTGTGCCAGCMGCCGCGGTAA TCATATGCCGGACTACHVGGGTWTCTAAT French Guiana 3 Gut 16s Anoplotermes banksi Normal 3 3 FG_Anoplo_SF_191120_1 Anp_Sf_1 TCCTTAGTGTGCCAGCMGCCGCGGTAA TAATGAGCCGGACTACHVGGGTWTCTAAT French Guiana 1 Soil food substrate 16s Anoplotermes banksi Not applicable 1 1 FG_Anoplo_SF_191120_2 Anp_Sf_2 TCAATACGTGTGCCAGCMGCCGCGGTAA TTAAGAACCGGACTACHVGGGTWTCTAAT French Guiana 2 Soil food substrate 16s Anoplotermes banksi Not applicable 1 1 FG_Anoplo_SF_191120_3 Anp_Sf_3 TCCATCTGTGTGCCAGCMGCCGCGGTAA TAACTCTCCGGACTACHVGGGTWTCTAAT French Guiana 3 Soil food substrate 16s Anoplotermes banksi Not applicable 2 2 FG_Anoplotermes_nest_190213_1 Anp_Ne_1 TGCGTTAGTGTGCCAGCMGCCGCGGTAA TTCGTAACCGGACTACHVGGGTWTCTAAT French Guiana 1 Nest 16s Anoplotermes banksi Not applicable 5 5 FG_Anoplotermes_nest_190213_2 Anp_Ne_2 TCTCGCAGTGTGCCAGCMGCCGCGGTAA TAGGTAGCCGGACTACHVGGGTWTCTAAT French Guiana 2 Nest 16s Anoplotermes banksi Not applicable 1 1 FG_Anoplotermes_nest_190213_3 Anp_Ne_2 TTAACCAGTGTGCCAGCMGCCGCGGTAA TCACTTGCCGGACTACHVGGGTWTCTAAT French Guiana 3 Nest 16s Anoplotermes banksi Not applicable 1 1 FG_Nasuti_GUTS_Ws_1 Nas_Gu_Ws_1 TCGCAGTGTGCCAGCMGCCGCGGTAA TGAGTCCGGACTACHVGGGTWTCTAAT French Guiana 1 Gut 16S Nasutitermes sp. Small 3 3 FG_Nasuti_GUTS_Ws_2 Nas_Gu_Ws_2 TTGACCTGTGTGCCAGCMGCCGCGGTAA TTAGATGCCGGACTACHVGGGTWTCTAAT French Guiana 2 Gut 16S Nasutitermes sp. Small 2 2 FG_Nasuti_GUTS_Ws_3 Nas_Gu_Ws_3 TAGACATGTGTGCCAGCMGCCGCGGTAA TTGACAACCGGACTACHVGGGTWTCTAAT French Guiana 3 Gut 16S Nasutitermes sp. Small 2 2 FG_Nasuti_NEST_200207_1 Nas_Ne_1 TACCGTCGTGTGCCAGCMGCCGCGGTAA TACAAGGCCGGACTACHVGGGTWTCTAAT French Guiana 1 Nest 16s Nasutitermes sp. Not applicable 6 6 FG_Nasuti_NEST_200207_2 Nas_Ne_2 TAGTGATGTGTGCCAGCMGCCGCGGTAA TAGGATTCCGGACTACHVGGGTWTCTAAT French Guiana 2 Nest 16s Nasutitermes sp. Not applicable 2 2 FG_Nasuti_NEST_200207_3 Nas_Ne_3 TAGACTAGTGTGCCAGCMGCCGCGGTAA TACGCATCCGGACTACHVGGGTWTCTAAT French Guiana 3 Nest 16s Nasutitermes sp. Not applicable 2 2 FG_Nasuti_Wbig_Guts_1 Nas_Wb_Gu_1 TCTCCTCGTGTGCCAGCMGCCGCGGTAA TTCACTACCGGACTACHVGGGTWTCTAAT French Guiana 1 Gut 16S Nasutitermes sp. Big 2 2 FG_Nasuti_Wbig_Guts_2 Nas_Wb_Gu_2 TAGGAGTGTGCCAGCMGCCGCGGTAA TACGTTCCGGACTACHVGGGTWTCTAAT French Guiana 2 Gut 16S Nasutitermes sp. Big 5 5 FG_Nasuti_Wbig_Guts_3 Nas_Wb_Gu_3 TCTACGAGTGTGCCAGCMGCCGCGGTAA TTGAATGCCGGACTACHVGGGTWTCTAAT French Guiana 3 Gut 16S Nasutitermes sp. Big 1 1 FG_Nasuti_WF_201030_1 Nas_Wf_1 TCGAGCAGTGTGCCAGCMGCCGCGGTAA TGCCAGTCCGGACTACHVGGGTWTCTAAT French Guiana 1 Wood food substrate 16s Nasutitermes sp. Not applicable 5 5 FG_Nasuti_WF_201030_2 Nas_Wf_2 TGTTCATGTGTGCCAGCMGCCGCGGTAA TACAGGACCGGACTACHVGGGTWTCTAAT French Guiana 2 Wood food substrate 16s Nasutitermes sp. Not applicable 2 2 FG_Nasuti_WF_201030_3 Nas_Wf_3 TTAGGTAGTGTGCCAGCMGCCGCGGTAA TAGATCTCCGGACTACHVGGGTWTCTAAT French Guiana 3 Wood food substrate 16s Nasutitermes sp. Not applicable 5 5 NTC_OLD1 NTC_OLD1 TAGACGCGTGTGCCAGCMGCCGCGGTAA TTCCAATCCGGACTACHVGGGTWTCTAAT Czech Republic 1 Negative control 16s Negative control Not applicable 1 1 NTC_OLD2 NTC_OLD2 TGGTGTTGTGTGCCAGCMGCCGCGGTAA TACCATACCGGACTACHVGGGTWTCTAAT Czech Republic 2 Negative control 16s Negative control Not applicable 1 1 PNG_Microcero_GUTS Mic_Gu TCGTCATGTGTGCCAGCMGCCGCGGTAA TTCTCATCCGGACTACHVGGGTWTCTAAT Papua New Guinea 1 Gut 16s Microcerotermes sp. Normal 2 2 PNG_Microcero_WF_190611 Mic_Wf TCATAGTGTGTGCCAGCMGCCGCGGTAA TGAATGTCCGGACTACHVGGGTWTCTAAT Papua New Guinea 1 Wood food substrate 16s Microcerotermes sp. Not applicable 3 3 PNG_Nasuti_GUTS Nas_Gu TGGTACAGTGTGCCAGCMGCCGCGGTAA TGACCTGCCGGACTACHVGGGTWTCTAAT Papua New Guinea 1 Gut 16s Nasutitermes sp. Normal 3 3 PNG_Nasuti_WF_181218 Nas_Wf TCTGAGCGTGTGCCAGCMGCCGCGGTAA TGAACAGCCGGACTACHVGGGTWTCTAAT Papua New Guinea 1 Wood food substrate 16s Nasutitermes sp. Not applicable 6 6 PNG_nest Mic_Ne TACGCTCGTGTGCCAGCMGCCGCGGTAA TCTCGCACCGGACTACHVGGGTWTCTAAT Papua New Guinea 1 Nest 16s Microcerotermes sp. Not applicable 2 2 CAM_Cephalo_GUTS_1_ITS Cep_Gu_1 TGGAATGCGTGARTCATCGARTCTTTG TGTCGTACTCCTCCGCTTATTGATATGC Cameroon 1 Gut ITS Cephalotermes sp. Normal 3 3 CAM_Cephalo_GUTS_2_ITS Cep_Gu_2 TACATAATGCGTGARTCATCGARTCTTTG TGTAGTTACTCCTCCGCTTATTGATATGC Cameroon 2 Gut ITS Cephalotermes sp. Normal 8 8 CAM_Cephalo_GUTS_3_ITS Cep_Gu_3 TAATTGTTGCGTGARTCATCGARTCTTTG TGGCTTACTCCTCCGCTTATTGATATGC Cameroon 3 Gut ITS Cephalotermes sp. Normal 4 4 CAM_Cephalo_Nest_190716_1_ITS Cep_Ne_1 TTCAATCAGCGTGARTCATCGARTCTTTG TCTCGTCTACTCCTCCGCTTATTGATATGC Cameroon 1 Nest ITS Cephalotermes sp. Not applicable 7 7 CAM_Cephalo_Nest_190716_2_ITS Cep_Ne_2 TAACGGATGCGTGARTCATCGARTCTTTG TTCCTCTCACTCCTCCGCTTATTGATATGC Cameroon 2 Nest ITS Cephalotermes sp. Not applicable 4 4 CAM_Cephalo_Nest_190716_3_ITS Cep_Ne_3 TTAACAATGCGTGARTCATCGARTCTTTG TACTCTACTCCTCCGCTTATTGATATGC Cameroon 3 Nest ITS Cephalotermes sp. Not applicable 4 4 CAM_Cephalo_WF_190719_1_ITS Cep_Wf_1 TAATAAGTGCGTGARTCATCGARTCTTTG TGGTCGTTACTCCTCCGCTTATTGATATGC Cameroon 1 Wood food substrate ITS Cephalotermes sp. Not applicable 8 8 CAM_Cephalo_WF_190719_2_ITS Cep_Wf_2 TTGGAATTGCGTGARTCATCGARTCTTTG TCTCACACTCCTCCGCTTATTGATATGC Cameroon 2 Wood food substrate ITS Cephalotermes sp. Not applicable 4 4 CAM_Cephalo_WF_190719_3_ITS Cep_Wf_3 TCACCACTGCGTGARTCATCGARTCTTTG TCCTCCACTCCTCCGCTTATTGATATGC Cameroon 3 Wood food substrate ITS Cephalotermes sp. Not applicable 8 8 CAM_Cubi_GUTS_ITS Cub_Gu TAGTGACTGCGTGARTCATCGARTCTTTG TTAGAACGACTCCTCCGCTTATTGATATGC Cameroon 1 Gut ITS Cubitermes sp. Normal 4 4 Cam_Cubi_nest_190430_ITS Cub_Ne TCGCTTGTGCGTGARTCATCGARTCTTTG TACTGGTACTCCTCCGCTTATTGATATGC Cameroon 1 Nest ITS Cubitermes sp. Not applicable 3 3 CAM_SF_Cubitermes_ITS Cub_Sf TTAGACTTGCGTGARTCATCGARTCTTTG TTGTCTATACTCCTCCGCTTATTGATATGC Cameroon 1 Soil food substrate ITS Cubitermes sp. Not applicable 6 6 FG_Anoplo_GUTS_1_ITS Anp_Gu_1 TAAGTAATGCGTGARTCATCGARTCTTTG TACAACACACTCCTCCGCTTATTGATATGC French Guiana 1 Gut ITS Anoplotermes banksi Normal 3 3 FG_Anoplo_GUTS_2_ITS Anp_Gu_2 TGTATGGTGCGTGARTCATCGARTCTTTG TACGAACCACTCCTCCGCTTATTGATATGC French Guiana 2 Gut ITS Anoplotermes banksi Normal 6 6 FG_Anoplo_GUTS_3_ITS Anp_Gu_3 TCTTGACTGCGTGARTCATCGARTCTTTG TTCCGTACTCCTCCGCTTATTGATATGC French Guiana 3 Gut ITS Anoplotermes banksi Normal 6 6 FG_Anoplo_SF_191120_1_ITS Anp_Sf_1 TACCTCTGCGTGARTCATCGARTCTTTG TCGTCTCCACTCCTCCGCTTATTGATATGC French Guiana 1 Soil food substrate ITS Anoplotermes banksi Not applicable 3 3 FG_Anoplo_SF_191120_2_ITS Anp_Sf_2 TATAAGATGCGTGARTCATCGARTCTTTG TTACCTACACTCCTCCGCTTATTGATATGC French Guiana 2 Soil food substrate ITS Anoplotermes banksi Not applicable 8 8 FG_Anoplo_SF_191120_3_ITS Anp_Sf_3 TTCAGCTTGCGTGARTCATCGARTCTTTG TGTGCCTACTCCTCCGCTTATTGATATGC French Guiana 3 Soil food substrate ITS Anoplotermes banksi Not applicable 7 7 FG_Anoplotermes_nest_190213_1_ITS Anp_Ne_1 TTGTTAGTGCGTGARTCATCGARTCTTTG TACCTACTACTCCTCCGCTTATTGATATGC French Guiana 1 Nest ITS Anoplotermes banksi Not applicable 3 3 FG_Anoplotermes_nest_190213_2_ITS Anp_Ne_2 TATAAGATGCGTGARTCATCGARTCTTTG TTACCTACACTCCTCCGCTTATTGATATGC French Guiana 2 Nest ITS Anoplotermes banksi Not applicable 4 4 FG_Anoplotermes_nest_190213_3_ITS Anp_Ne_3 TACCTCTGCGTGARTCATCGARTCTTTG TCGTCTCCACTCCTCCGCTTATTGATATGC French Guiana 3 Nest ITS Anoplotermes banksi Not applicable 7 7 FG_Nasuti_GUTS_Ws_1_ITS Nas_Gu_Ws_1 TAATGAATGCGTGARTCATCGARTCTTTG TAGTAACTACTCCTCCGCTTATTGATATGC French Guiana 1 Gut ITS Nasutitermes sp. Small 7 7 FG_Nasuti_GUTS_Ws_2_ITS Nas_Gu_Ws_2 TTCTATGCGTGARTCATCGARTCTTTG TCCACTACTCCTCCGCTTATTGATATGC French Guiana 2 Gut ITS Nasutitermes sp. Small 7 7 FG_Nasuti_GUTS_Ws_3_ITS Nas_Gu_Ws_3 TCCGTTGCGTGARTCATCGARTCTTTG TACGATACTCCTCCGCTTATTGATATGC French Guiana 3 Gut ITS Nasutitermes sp. Small 8 8 FG_Nasuti_NEST_200207_1_ITS Nas_Ne_1 TAACGACTGCGTGARTCATCGARTCTTTG TACCTGCCACTCCTCCGCTTATTGATATGC French Guiana 1 Nest ITS Nasutitermes sp. Not applicable 8 8 FG_Nasuti_NEST_200207_2_ITS Nas_Ne_2 TACATGGTGCGTGARTCATCGARTCTTTG TGTCTCACTCCTCCGCTTATTGATATGC French Guiana 2 Nest ITS Nasutitermes sp. Not applicable 4 4 FG_Nasuti_NEST_200207_3_ITS Nas_Ne_3 TTCCTTGCGTGARTCATCGARTCTTTG TGTATGCCACTCCTCCGCTTATTGATATGC French Guiana 3 Nest ITS Nasutitermes sp. Not applicable 4 4 FG_Nasuti_Wbig_Guts_1_ITS Nas_Gu_Wb_1 TACATTCTGCGTGARTCATCGARTCTTTG TTCCGACTACTCCTCCGCTTATTGATATGC French Guiana 1 Gut ITS Nasutitermes sp. Big 8 8 FG_Nasuti_Wbig_Guts_21_ITS Nas_Gu_Wb_2 TATACGGTGCGTGARTCATCGARTCTTTG TGTACGATACTCCTCCGCTTATTGATATGC French Guiana 2 Gut ITS Nasutitermes sp. Big 7 7 FG_Nasuti_Wbig_Guts_3_ITS Nas_Gu_Wb_3 TCATGGTTGCGTGARTCATCGARTCTTTG TCCTTATTACTCCTCCGCTTATTGATATGC French Guiana 3 Gut ITS Nasutitermes sp. Big 8 8 FG_Nasuti_WF_201030_1_ITS Nas_Wf_1 TCTACTGCGTGARTCATCGARTCTTTG TAGACGACTCCTCCGCTTATTGATATGC French Guiana 1 Wood food substrate ITS Nasutitermes sp. Not applicable 3 3 FG_Nasuti_WF_201030_2_ITS Nas_Wf_2 TGTCGTTGCGTGARTCATCGARTCTTTG TGCCTATTACTCCTCCGCTTATTGATATGC French Guiana 2 Wood food substrate ITS Nasutitermes sp. Not applicable 4 4 FG_Nasuti_WF_201030_3_ITS Nas_Wf_3 TCAAGGATGCGTGARTCATCGARTCTTTG TGGTACACACTCCTCCGCTTATTGATATGC French Guiana 3 Wood food substrate ITS Nasutitermes sp. Not applicable 7 7 NTC_OLD1_ITS NTC_OLD1 TACGTGTGCGTGARTCATCGARTCTTTG TATACACTACTCCTCCGCTTATTGATATGC Czech Republic 1 Negative ITS Negative control Not applicable 1 1 PNG_Microcero_GUTS_ITS Mic_Gu TCCATTATGCGTGARTCATCGARTCTTTG TGTTCTCTACTCCTCCGCTTATTGATATGC Papua New Guinea 1 Gut ITS Microcerotermes sp. Normal 2 2 PNG_Microcero_WF_190611_ITS Mic_Wf TCCTGTGCGTGARTCATCGARTCTTTG TTGTCCGTACTCCTCCGCTTATTGATATGC Papua New Guinea 1 Wood food substrate ITS Microcerotermes sp. Not applicable 8 8 PNG_Nasuti_GUTS_ITS Nas_Gu TACGACTTGCGTGARTCATCGARTCTTTG TTACAACACTCCTCCGCTTATTGATATGC Papua New Guinea 1 Gut ITS Nasutitermes sp. Normal 7 7 PNG_Nasuti_WF_181218_ITS Nas_Wf TGCTGCCTGCGTGARTCATCGARTCTTTG TCCAGTCTACTCCTCCGCTTATTGATATGC Papua New Guinea 1 Wood food substrate ITS Nasutitermes sp. Not applicable 4 4 PNG_nest_ITS Mic_Ne TATTCTGCGTGARTCATCGARTCTTTG TAGTGGCTACTCCTCCGCTTATTGATATGC Papua New Guinea 1 Nest ITS Microcerotermes sp. Not applicable 7 7