A direct electrode-driven P450 cycle for biocatalysis
收藏PubMed Central1997-12-09 更新2026-05-02 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC28344/
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资源简介：
The large potential of redox enzymes to carry out formation of high value organic compounds motivates the search for innovative strategies to regenerate the cofactors needed by their biocatalytic cycles. Here, we describe a bioreactor where the reducing power to the cycle is supplied directly to purified cytochrome CYP101 (P450cam; EC 1.14.15.1) through its natural redox partner (putidaredoxin) using an antimony-doped tin oxide working electrode. Required oxygen was produced at a Pt counter electrode by water electrolysis. A continuous catalytic cycle was sustained for more than 5 h and 2,600 enzyme turnovers. The maximum product formation rate was 36 nmol of 5-exo-hydroxycamphor/nmol of CYP101 per min.
提供机构：
National Academy of Sciences
创建时间：
1997-12-09
