The impact of conditional Opa1 Knockout in neural stem cells on transcriptome changes. The impact of conditional Opa1 Knockout in neural stem cells on transcriptome changes

To investigate the function of Opa1 in the regulation of adult neurogenesis, we created a conditional Opa1-knockout mouse line by cross-breeding Nestin-CreERT2;ROSA26YFP mice with Opa1-Flox mice. Using Tamoxifen, we induced Opa1 knockout on 8-weeks-old Nestin-CreERT2;ROSA26YFP;Opa1-Flox mice. We then sorted YFP-positive cells from the hippocampus using flow cytometry at 10 weeks. RNA was extracted from sorted cells and subjected to next generation sequencing. Overall design: Comparative gene expression profiling analysis of RNA-seq data for WT and Opa1-KO neural stem cells.