Additional file 10: of Alternative polyadenylation produces multiple 3’ untranslated regions of odorant receptor mRNAs in mouse olfactory sensory neurons

Table S4. Alternative 3′ ends for pilot genes annotated from RNA-Seq data and RL-PAT experimental validation. The 3′ end positions and the relative abundances of the resulting 3’UTR isoforms were obtained using our IsoSCM workflow to process the 4 datasets from adult OM. The 3′ ends distant of less than 100 nt were merged as a single polyA sitea. For each putative polyA site, predicted canonical PASs matching in a [− 100;+ 100] window from the RNA-Seq-deduced 3′ end are indicatedb. Note that for Olfr1507 3’XL isoform, the closest canonical PAS is slightly outside the 100-nt precision windowc. The cleavage site and polyA tail length were experimentally mapped using RL-PAT on adult male OM, further confirming the polyA site usage for most of the 3’UTR isoforms. XL site of Olfr1507, M1 and L sites of Olfr15 were not confirmed by RL-PAT since no specific forward primer could be designed due to repeated sequences upstream of these polyA sitesd. For each confirmed polyA site, predicted canonical PASs, or variant hexamers when necessary, matching in a [− 100;0] window from the identified cleavage sites are indicatede. ND: not detected; NA: not available. (XLSX 39 kb)