Metagenomic next-generation sequencing reveals difference about microbiota and immune status in patients with different lung infections. Metagenomic next-generation sequencing reveals difference about microbiota and immune status in patients with different lung infections

We used Metagenomic next-generation sequencing approach to compare the differences between the three pneumonias in terms of immune-related genes and interferon-stimulated genes with the microbiota. GSEA enrichment analysis was used to analyze pathways to confirm these observations. The composition and abundance of immune cells were estimated using CIBERSORT in mixed cells.Differential species between different groups were detected using the rank sum test and downscaled by linear discriminant analysis. The final results were obtained by associating differential immune-related genes by differential species.