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Accuracy, precision and resolution of methods to distinguish rBet v 1a conformation-dependent IgE binding and secondary structure of rBet v 1a/rBet v 1a<sub>S112P/R145P</sub> mixtures.

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1analytical resolution between 100% and x% Bet v 1a in Bet v 1a/Bet v 1aS112P/R145P mixtures 2in deg*cm2*dmol-1 3arbitrary units 4inhibitor concentration (ng/well) for half-maximum inhibition of IgE binding 5protein concentration (ng/well) for half-maximum mediator release CD222nm: mean residual ellipticiy at 222 nm; IB: immunoblot; ELISA: Inhibition-Enzyme-linked immunosorbent assay; RBL: cellular mediator release with humanized rat basophilic leukemia cells Accuracy, precision and resolution of methods to distinguish rBet v 1a conformation-dependent IgE binding and secondary structure of rBet v 1a/rBet v 1aS112P/R145P mixtures.

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2015-07-17
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