SLIRP mediated rRNA 2'-O-methylation

SLIRP is a versatile protein that can interact with stem-loop structure in RNA and with G quadruplex DNA. By using a quantitative proteomic experiment, we found that SLIRP interacts with the majority of the human helicase proteome. We found that these interactions facilitate 2'-O-methylation of rRNA and promote protein translation. Hence, we uncovered a novel function of SLIRP protein and offered novel mechanistic insights into its function as a RNA chaperone and into the regulation of 2'-O-methylation of rRNA. Overall design: We performed the RiboMethSeq in 293T cells with or without knockdown of SLIRP. Three replicates were done for each experiments.