Sexual recombination under tetrapolar mating can alter host-specialization boundaries between wheat- and barley-adapted stripe rust lineages

Haplotype-resolved nuclear genome assemblies and associated resources for mating-type locus analyses in Puccinia striiformis f. sp. tritici DK02d_12 (PstS8) and P. striiformis f. sp. hordei NP85002 (Psh). The dataset includes genome assemblies, phylogenetic tree files, mating-type locus coding sequences, and regional GFF3 coordinate files containing predicted gene and transposable element features used as annotation tracks for PR locus synteny visualization. The Pst104E PR locus files are included as a reference for comparative synteny analysis. 1. Genome assemblies: Pst_DK02d_12.fasta (both haplotypes combined) Pst_DK02d_12_hap1.fasta (haplotype 1) Pst_DK02d_12_hap2.fasta (haplotype 2) 2. Phylogenetic tree files: Puccinia_species_tree_alignment.fa.treefile (Genome-wide orthology) HD_bW_raw.tree (homeodomain (HD) locus bW) HD_bE_raw.tree (homeodomain (HD) locus bE) 3. HD and PR coding sequences: Pst_bW_cds.fasta Pst_bE_cds.fasta Psh_PRA.fasta 4. PR locus regional feature tracks: Psh_chr6_1.gene.gff3 Psh_chr6_1.TE.gff3 Psh_chr6_2.gene.gff3 Psh_chr6_2.TE.gff3 PstS8_chr6_1.gene.gff3 PstS8_chr6_1.TE.gff3 PstS8_chr6_2.gene.gff3 PstS8_chr6_2.TE.gff3 Pst104E_chr6_1.gene.gff3 Pst104E_chr6_1.TE.gff3 Pst104E_chr6_2.gene.gff3 Pst104E_chr6_2.TE.gff3