Transcriptomic Analysis by RNA-seq in a WSU-FSCCL Cell Line Model of Lymphoma Malignant Progression

We performed single-cell RNA sequencing on mice with an established lymphoma model and identified two distinct cell clusters in the lymph nodes exhibiting prominent malignant progression characteristics. Pseudotime analysis revealed a clear trajectory of malignant progression in these clusters. Based on these findings, we developed a model correlating the expression levels (high/low) of signature genes from the WSU-FSCCL cell line with these characteristic subpopulations and subsequently conducted corresponding RNA-seq analyses.