FGF19-FGFR4 promotes the progression of gallbladder carcinoma in a biliary autocrine pathway dependent on GPBAR1-EGR1 axis

GBC is the most common type of biliary tract cancers and the seventh most common gastrointestinal cancer. Most patients are diagnosed as GBC in an unresectable stage because of the silent symptoms. Even after curative surgery, most patients undergo recurrence due to the early metastasis and limited adjuvant therapies. All these reasons lead to an extremely dismal prognosis of advanced GBC, with a 5-year survival rate approximately 5%-10%. Cholestasis has been shown to be an important factor in the malignant progression of gallbladder cancer. In this study, we stimulate GBC-SD cells with high concentration of CDCA to simulate cholestasis and analyzed the DEGs in the stimulation of CDCA. Overall design: GBC-SD cells were stimulated with CDCA(100µM) for 72 hours and subjected to differential gene expression studies by RNA-seq