OPTIMIZATION OF PROCESS PARAMETERS FOR THE PRODUCTION OF GLUTAMINASE ENZYME FROM ERWINIAAT FLASK LEVEL.

This purpose of study was to determine parameter optimization for the<br>production of Glutaminase enzyme from Erwinia at flask level. This study was carried<br>out at Department of Biotechnology, Mata Gujri College, Fatehgarh Sahib, Punjabi<br>University Patiala For maximum production of glutaminase from free cells of Erwinia,<br>various physical and chemical parameters were optimized. The microorganism Erwinia<br>which is a source of glutaminase was procured from Institute of Microbial Technology,<br>Chandigarh and was grown on specified medium. The media was sterilized by<br>autoclaving for 15 minutes at 15 psi/cm2. The microorganism was maintained on agar<br>slants and kept in refrigerator at 4.0 + 0.5 0C for further use and was sub-cultured after<br>every 20 days. The viability check of microorganism was done by gram staining and<br>tryphan blue. The work utilized Beef extract, Peptone, Yeast extract, Sodium chloride<br>and Water as composition of growth medium for the production of glutaminase enzyme<br>from Erwinia. The parameters such as Temp., Ph, Time and Inducer concentration were<br>optimized.The enzyme activity was determined by Nessler's reagent method and protein<br>concentration was determined by Bradford method. It was concluded that Maximum<br>production of glutaminase enzyme from Erwinia was observed at temp.-25?C, pH-6.5 &amp;<br>Time after-48 hours. At these optimized parameters enzyme activity and Protein<br>concentration was found maximum