BRED frequencies for constructing deletions in the tape measure gene of mycobacteriophage Giles.
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1Mutants generated have in-frame deletions in Giles gene 20; deletion sizes in base pairs (bp).2Substrates were either oligonucleotides (name-length) or dsDNA as indicated.3Plaques recovered from electroporation analyzed by PCR as indicated; NT, not tested.4Substrate also incorporates point mutations; frequencies reflect deletion formation only.
创建时间:
2015-12-02



