Effects of m6A modification on turnover of newly synthesized mRNAs at the transcriptome level.

We developed a method to deconvolute decays of m6A mRNAs and their unmethylated isoforms from newly synthesized total RNAs across the transcriptome to study the effects of m6A modification on decays of not only the m6A-modified RNA pool but also the unmehnylated isoform pool. Overall design: M6A-RIP-seq was combined with time-course experiments using BrU-mediated metabolic labeling approach to monitor how decays of newly synthesized m6A mRNA and unmethylated isoform mRNA pools may be different at the transcriptome level. Thirty-six RNA samples were generated from two biological repeats.