Establishing murine trophoblast organoids as a platform for CRISPR-Cas9 screening [scRNA-seq]

Murine trophoblast organoids contain trophoblast cell types. We used single cell RNA sequencing (scRNA-seq) to analyze the cellular composition of trophoblast organoids derived from placentae (mPOs). Overall design: Trophoblast organoids in the maintenance condition and the differentiation condition were digested into single cells by accutase and papain, respectively. The libraries were constructed on the 10X Genomics. Library samples were subjected to Illumina Nova-seq 6000 sequencing system with a sequencing depth of at least 50,000 reads per cell and 150-bp (PE150) paired-end reads.