Morin hydrate reduces fertility and survival, delays development, and weakens lipid reserves in Aedes aegypti

The Aedes aegypti mosquito is generally associated with arboviruses that cause yellow fever, dengue, zika and chikungunya. The most efficient way to control their populations is through application in breeding sites of highly toxic insecticides that can also impact non-target organisms and generate resistant populations. Therefore, the use of compounds is desirable. Morin hydrate has broad pharmacological applications based on its antioxidant potential, in addition to not having negative effects on mammals. Therefore, the objective of the present study was to investigate the effects of morin hydrate on A. aegypti survival, pupation rate, egg laying, triacylglycerol reserves, and expression of proteins related to lipid metabolism 24 h after exposure of larvae. For this, rearing media containing A. aegypti larvae with different concentrations of morin hydrate were formulated to evaluate the lethal concentration. Calculation of the expected lethal concentrations showed LC25 of 52.692 µM, L..., Mosquito Rearing
The study utilized Aedes aegypti (Rockefeller strain) larvae from a laboratory colony maintained under controlled conditions (27±1°C, 80±10% relative humidity, 12h light/dark cycle). Larvae were fed commercial cat food, while adults received a 10% sucrose solution. Blood meals were provided to 4-day-old females for egg production. All procedures followed ethical guidelines approved by CEUA/UFRRJ (protocol #006/2022).
Chemical Exposure
Third-instar larvae were exposed to morin hydrate (10-2500 µM) dissolved in 0.1% ethanol for 24 hours under fasting conditions. A control group received only the 0.1% ethanol vehicle. Larval density was maintained at 1 individual per mL of solution throughout the experiments.
Biological Assessments
Mortality was evaluated after 24-hour exposure across four replicates (10 larvae/concentration). For chronic effects, surviving larvae were transferred to clean water and monitored for pupation and adult emergence over six days. Adult fecundity ..., , # Morin hydrate reduces fertility and survival, delays development, and weakens lipid reserves in Aedes aegypti

Dataset DOI: [10.5061/dryad.x0k6djhwh](10.5061/dryad.x0k6djhwh)

## Description of the data and file structure

**Dataset Overview:**

This dataset contains experimental data from Santos et al. (2025), testing the hypothesis that morin hydrate disrupts lipid metabolism in *Aedes aegypti* larvae, encompassing behavioral assays and molecular analyses. The dataset includes larval mortality data with LC25 (52.692 µM), LC50 (174.775 µM), and LC90 (1685.936 µM) values derived from dose-response curves. Survivors exhibited a 2-fold delay in pupation time and an approximately 30% reduction in egg-laying by females exposed to concentrations above 100 µM. Biochemical analyses revealed dose-dependent triacylglycerol depletion (reductions of 17%, 33%, and 61% at 100, 175, and 500 µM, respectively), which correlated with alterations in lipid metabolism gene expression, upregulation of lip...,