EDNA data for Crown of Thorns Seastar (Acanthaster cf. solaris)
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AIMS developed an eDNA based methods to detect and quantify CoTS. The data included here include field data from initial method development as part of AIMS appropriation, and later testing through several CoTS Control Innovation Program (CCIP) projects. Data consist of filtered water samples, in most cases collected with 12 replicates at three sites per reef. Data Dictionary:Field name: Explanation/unitsDate: Julian DateYear: Collection YearReef: GBR Reef NameSite_name: Site nameSite_No: Site numberLat: Latitude in decimal degreesLong: Longitude in decimal degreesProject: Project CodeReef_rep: replicate number on a reef basisSite_rep: replicate number on a sitePCR1_binary: First technical PCR replicate: positive or negative (1 or 0)PCR2_binary: First technical PCR replicate: positive or negative (1 or 0)LOD_sample_positive: Limit of Detection based: 1 if either of the technical replicates are positivePCR1_conc: CoTS eDNA copy number per L water filtered, first technical replicatePCR2_conc: CoTS eDNA copy number per L water filtered, second technical replicateConc_mean: CoTS eDNA copy number per L water filtered, averaged over both replicates



