Structure of human TRPM8 channel

This repository includes the following source datasets:

1. modeling.zip
Modeling of icilin binding to HsTRPM8 (Figure 3e, 3f, 3g, 3h)
 - HsTRPM8-KX7_opt.pdb: original pose (Figure 3e, 3f)
 - HsTRPM8-KX7_opt_rotated.pdb: rotates pose (Figure 3g, 3h)

2. activation_assay.zip
HsTRPM8 activation assay results (Figure 3i and Supplementary Figure 5)
 - HsTRPM8_activation_assay_results.pzf: GraphPad file with activation Assay results

3. Fr-TM.zip
Structure-based multiple sequence alignment of TRPM channels (Supplementary Figure 4a)
 - Fr-TM_info.txt: description of procedure used to create alignment
 - TRPM_new_raw_pairwise_alignments.fas: raw pairwise alignment of new TRPM sequences to 6CO7 reference
 - TRPM_S6_restriction_site_aligned.fas: final multiple sequence alignment used to generate Supplementary Figure 4a

4. 3Dvar.zip
HsTRPM8 3D Variability Analysis results (Supplementary Movie 1)
 - *.mrc files: composite maps with extreme frames for two analyzed components (aligned with focused MHR1/2 map)
 - *.pdb files: HsTRPM8 models fitted (MDFF) to above maps and used to generate Supplementary Movie 1

5. Supplementary_Movie_1.mp4
Morphing between different conformations of HsTRPM8 resolved by 3D Variability analysis (Supplementary Movie 1).