Structure of human TRPM8 channel

This repository includes the following source datasets: 1. modeling.zip Modeling of icilin binding to HsTRPM8 (Figure 3e, 3f, 3g, 3h) - HsTRPM8-KX7_opt.pdb: original pose (Figure 3e, 3f) - HsTRPM8-KX7_opt_rotated.pdb: rotates pose (Figure 3g, 3h) 2. activation_assay.zip HsTRPM8 activation assay results (Figure 3i and Supplementary Figure 5) - HsTRPM8_activation_assay_results.pzf: GraphPad file with activation Assay results 3. Fr-TM.zip Structure-based multiple sequence alignment of TRPM channels (Supplementary Figure 4a) - Fr-TM_info.txt: description of procedure used to create alignment - TRPM_new_raw_pairwise_alignments.fas: raw pairwise alignment of new TRPM sequences to 6CO7 reference - TRPM_S6_restriction_site_aligned.fas: final multiple sequence alignment used to generate Supplementary Figure 4a 4. 3Dvar.zip HsTRPM8 3D Variability Analysis results (Supplementary Movie 1) - *.mrc files: composite maps with extreme frames for two analyzed components (aligned with focused MHR1/2 map) - *.pdb files: HsTRPM8 models fitted (MDFF) to above maps and used to generate Supplementary Movie 1 5. Supplementary_Movie_1.mp4 Morphing between different conformations of HsTRPM8 resolved by 3D Variability analysis (Supplementary Movie 1).