SREBF1 coordinates with master transcription factors in regulating lipid metabolism and cancer-promoting pathways in squamous cell carcinoma [4C]

To determine whether a TP63/KLF5-regulated super-enhancer region can impact SREBF1 transcription, circularized chromosome conformation capture (4C) assays were performed. 4C assays identified complex, extensive interactions between the SREBF1 promoter and the super-enhancer region Moreover, these DNA-DNA contacts were strictly confined within the super-enhancer region, highlighting the specificity of chromatin interactions Overall design: We performed Circularized chromosome conformation capture (4C) assays in TE5 cells using the SREBF1 promoter as bait to measure the direct contact between the super-enhancer and SREBF1 promoter. The viewpoint of SREBF1 promoter region was chr17:17738740-17740085 (hg19) with 4C primers: NEST-Forward-GCAACCAGCTGGGCTCAT, OUTER-Forward-GAAGCAACGGGCCTCCTAAT, NEST-Reverse-CTGCTGACCGACATCGAAGG and OUTER-Reverse-TTGCGAGGTTACTCACGGTC