Number of animals in the treatment groups used for developmental milestones and behavioral testing.

Note: Numbers in the table represent number of animals per group for males and females. The first number indicates the number of pups assessed for developmental milestones, and the number in brackets indicates the number of animals that underwent behavioral testing. There were 3 litters in each of the 4 prenatal groups (2VEH, 5VEH: 2 or 5 injections of phosphate buffered saline vehicle on G15–16 or G12–16, respectively; LPS: Lipopolysaccharide, 50 ug/kg on G15–16; PPA: Propionic acid, 500 mg/kg on G12–16). Postnatal treatment during the second week of rat pups’ life consisted of phosphate buffered saline vehicle (VEH) or propionic acid (PPA). A maximum of 5 males and 5 females (3 postnatal PPA, 2 postnatal VEH for each sex) per litter were included in behavioral testing. Testing took place on P40 and 42.