single-cell RNA sequencing reveals the mechanism of human breast cancer metastasis

To obtain more information about the lymph node metastasis of breast cancer cells, we selected the matched primary breast cancer (PC), positive lymph nodes (PL), and negative lymph nodes (NL) of the same patient to perform integrated analysis. The PC, PL, NL samples were analysed with single-cell RNA sequencing. Overall design: The matched primary breast cancer (PC), positive lymph nodes (PL), and negative lymph nodes (NL) samples were analysed with single-cell RNA sequencing.