Gene expression in D8 WT and Menin KO uteri

Differential expression genes were normalized to fragments per kilobase of exon model per million mapped reads (RPKM) using EdgeR package in R with the criteria of fold change significantly greater than 1.5 and P<0.05. Gene ontology (GO) enrichment analysis of the DEGs was analyzed by using DAVID (the Database for Annotation, Visualization and Integrated Discovery, https://david.ncifcrf.gov). GO terms with corrected p <0.05 were considered significantly enriched. Gene Set Enrichment Analysis (GSEA) was performed to determine whether a defined set of genes shows statistically significant, consistent differences between Men1f/f and littermates Men1d/d decidual tissues. Total RNAs from D8 uteri were extracted by TRIzol and purified by poly-A before subjected to RNA-Seq. The expression of level of each gene is normalized to RPKM.