Early evidences of structural rearrangements in antibodies induced by chemical reduction of the hinge region

The experiment is aimed to verify the integrity and functionality of the antigen binding site in reduced antibodies. The data allow the estimation of the activity of HRP bound by whole and reduced antibodies, in such a way it is possible to estimate the antigen binding capacity of antibodies directed against HRP. The data reveals that not only the antigen binding capacity of reduced antibodies was retained, but the signal generated by these ones is higher than the signal generated by whole antibody.