CRISPR and biochemical screens Identify MAZ as a co-factor in CTCF-mediated insulation at Hox Clusters [ChIP-seq_set3]

In this experiment, we sought to identify how the distribution of CTCF change in WT, MAZ HoxA(?5|6), MAZ HoxD(?4|8), and CTCF (?5|6:6|7) cells during differentiation of mouse embryonic stem cells (ESCs) into cervical motor neurons (MNs). Overall design: Examination of CTCF binding in embryonic stem cells and cervical motor neurons using ChIP-seq, by status of motif deletion in the cells: WT, MAZ HoxA(?5|6), MAZ HoxD(?4|8), and CTCF (?5|6:6|7).