Images from Chandler, Chari et al. 2017 PLoS Genetics "How well do you know your mutation? Complex effects of genetic background on expressivity, complementation, and ordering of allelic effects"

These are the images associated with the data collected for <br><br>http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007075<br><br>For the zipped folder GS_data:<br>Data Provenance (how data was collected):<br>via dissections, area imaging<br>Author: <br>Performed - Ian Dworkin, Gayatri Sivaratnam, Samiksha Kaul <br>Dissections - Gayatri Sivaratnam, Samiksha Kaul<br>Imaging - I. Dworkin, G. Sivaratnam, Samiksha Kaul <br><br>Dates <br>samples Collected: July 21, 2015<br>Dissection: August 17 - September25, 2015<br>Imging: September 30, 2015 (ID, GS, SK)<br>(Date samples were collected, dates for imaging)<br><br>A bit about the experiment:<br>16 DGRP lines were crossed to 3 mutations (sd 1, sd E3, sd 58d) and a wildtype (SAM)<br>The effects of intermediate mutation on different lines, whether the severity of this effect varied across the different lines is being investigated.<br><br>Microscope, camera used. Leica m125 with a Leica DFC400 camera, using Leica Application Software 3.4.0. Greyscale images. <br><br>Each image is 1392 x 1040 px. Real size 2.59 x 1.93 mm<br><br>Magnification : 50X (0.5X?)<br>aperture about halfway across (59%)<br>Brightness: 68%<br>Gamma: 0.80<br><br>Notes:<br>naming convention for images: SAM/sd1/sde3/sd58d_line#_M_1/2/3/4/5/6.TIF<br><br>SAM/sd1/sde3/sd58d - either the wild type (SAM) allele for scalloped, or allele 1, E3 or 58d