Gene expression data from MGH-U3, RT112 and UM-UC-5 following TYRO3 knock-down. Homo sapiens

To better understand the molecular mechanisms underlying TYRO3 oncogenic activity in bladder carcinomas, we made use of MGH-U3, RT112 and UM-UC-5 cell lines, which were derived from a human bladder tumor and endogenously expressed the Tyro3 protein, the growth and transformation of these cell lines being dependent on Tyro3. We carried out a gene expression analysis using Affymetrix DNA arrays in this cell line treated or not withTYRO3 siRNAs. Overall design: MGH-U3, RT112 and UM-UC-5 cells were transfected or not with siRNA TYRO3. mRNA was extracted after 40h and gene expression analysis was assessed using the HG-U133 Plus 2.0 array (Affymetrix) array