Mechanistic insights into anti-cancer properties of Curcumin and Andrographis in colorectal cancer. Mechanistic insights into anti-cancer properties of Curcumin and Andrographis in colorectal cancer

Colorectal cancer (CRC) remains the major leading cause of cancer-related deaths worldwide. The limitations of current chemotherapeutic drugs in CRC include their toxicity, side effects, and exorbitant costs. To assess these unmet needs in CRC treatment, several naturally occurring compounds, including Curcumin and Andrographis, have gained increasing attention due to their multi-targeted functionality and safety vs. conventional drugs. In the current study, we revealed that a combination of Curcumin and Andrographis exhibited superior anti-tumor effects by inhibiting cell proliferation, invasion, colony formation, and induction of apoptosis. Genomewide transcriptomic expression profiling analysis revealed that Curcumin and Andrographis activated the ferroptosis pathway. Overall design: Identify differentially expressed genes in Curcumin or Andrographis treated cells compared to non-treated cells