Supplementary Material for: Soy Gly m 8 sIgE has limited value in the diagnosis of Soy Allergy in Peanut Ara h 2 Sensitised Adults

Background: Recently, specific IgE (sIgE) sensitization against Gly m 8 (soy 2S albumin) has been described as a good diagnostic marker for soy allergy (SA). The aim of this study is to evaluate the diagnostic value of Gly m 8 by determining the sensitization profiles based on the homologues soy allergens Bet v 1, Ara h 1, Ara h 2 and Ara h 3. Methods: Thirty soy allergic adults were included, sIgE to total soy extract, Gly m 8, Gly m 4, Gly m 5, Gly m 6, Bet v 1, Ara h 1, Ara h 2 and Ara h 3 were determined. Sensitization patterns were analyzed and determined. The clinical relevance of sIgE of Gly m 8 sensitization was measured by assessing its capacity to degranulate basophils in Gly m8 sensitized patients by an indirect Basophil Activation Test (iBAT). Results: Based on the sIgE patterns of sensitization two groups of SA patients were identified. (i) Peanut-associated SA group (all patients were sensitized to one or more of the peanut compounds) and (ii) Non-Peanut/PR-10-associated SA group (22 patients were sensitized to Gly m 4 and Bet v 1 but not to any of the peanut compounds). A high and significant correlation between total soy extract and Gly m 6 (R2 =0.97), Gly m 5 (R2 =0.85) and Gly m 8 (R2 =0.78) was observed. A non-significant correlation was observed between the levels of sIgE of Gly m 8 vs Ara h2. The iBAT results showed that Gly m 8 did not induce basophil degranulation in any of the Peanut-associated patients; indicating that the Gly m8 sensitizations were not clinically relevant. Conclusions: Gly m 8 was not a major allergen in the selected soy allergic population. The iBAT results indicated that Gly m 8 was not able to induce basophil degranulation in sIgE Gly m 8 sensitized soy allergic patients. Thus, Gly m 8 would have no added value in the diagnosis of SA in the present study population.