NOTCH Signaling Specifies a Transient Arterial-Type Hemogenic Endothelium that Gives Rise to Definitive-Type Hematopoiesis from Human Pluripotent Stem Cells

Recently, we identified and characterized specific endothelial progenitors with varying hemogenic potential during human pluripotent stem cell differentiation. Based on these studies we established a platform on which we can manipulate NOTCH signaling on these subsets to elucidate the specific role of this signaling pathway during hemogenic endothelial specification, endothelial-to-hematopoietic transition, and definitive hematopoietic specification. Overall design: Manipulate NOTCH signaling during hemogenic endothelial specification, endothelial-to-hematopoietic transition, and definitive hematopoietic specification and expansion.