Design, creation, and characterization of a stable, monomeric triosephosphate isomerase.
收藏PubMed Central1994-02-15 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC43190/
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资源简介：
Protein engineering on trypanosomal triosephosphate isomerase (TIM) converted this oligomeric enzyme into a stable, monomeric protein that is enzymatically active. Wild-type TIM consists of two identical subunits that form a very tight dimer involving interactions of 32 residues of each subunit. By replacing 15 residues of the major interface loop by another 8-residue fragment, a variant was constructed that is a stable and monomeric protein with TIM activity. The length, sequence, and conformation of the designed fragment were suggested by extensive modeling. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1994-02-15
