Spreading-dependent or independentSir2-mediated gene silencing in budding yeast

purpose: investigation the global insights into Sir2-dependent gene silencing on all silent chromatin regions in budding yeast Method: RNA-sequencing Results: the Sir2 binding domain length caused by Sir2 spreading from the chromosomal end is different in each telomere in budding yeast. Expression of most subtelomeric genes increased in the ∆sir2 strain. Some Sir2-regulated subtelomeric genes were positioned within the telomeric Sir2-binding domain, while the others were outside the Sir2-binding domain. In addition, Sir2 was bound to the mating-type loci and rDNA region, and gene expression increased in the ∆sir2 strain. Conclusion: that S. cerevisiae has two modes of Sir2-mediated gene silencing: One is dependent on chromatin binding and spreading of Sir2, and the other is independent of spreading of Sir2