Summary of significant metabolites differentiating between males and females in ob/ob mice.

This analysis employed three parameters, VIP score from OPLS-DA, p value in t-test, and relatively high metabolic concentrations between the genders, to identify the significant metabolites differentiating between the genders in obese group. If the VIP score and/or p value of a metabolite was altered significantly or its relatively high concentration was reversed between the genders, among the significant metabolites with VIP score >1 and p<0.05, the metabolite was listed above. For the VIP plot for all samples, see Figure S3. aThis indicates whether the metabolic concentration is relatively high in male or female. Also, the p values of the individual metabolic concentration in t-test are symbolized as *, **, and ***, indicating p<0.05, p<0.01, and p<0.005, respectively.