The deconvolution analysis of amide-I peaks was performed using the 4th derivative of the Gaussian-Laurentzian-normalized FTIR spectra as described in the Materials and Methods.
Secondary structure estimation was accomplished according to Yang's method (41). Percent secondary structure of lysozyme, RNase A and α-LA on modification by HTL.
The essential chaperonin TRiC/CCT mediates protein folding in cooperation with the co-chaperone prefoldin (PFD). As shown in vitro, the cylindrical TRiC complex facilitates folding through ATP-regulat
