[E-MTAB-131] Estrogen Receptor alpha estrogen-responsive binding sites in MCF-7

We performed chromatin immunoprecipitation (ChIP) with two polyclonal anti-ERalpha antibodies directed against the N- and C-terminus of the protein, with or without estrogen treatment for 45 minutes of MCF-7 cells, and the immunoprecipitated DNA was sequenced with the Illumina/Solexa Genome Analyzer. ArrayExpress Release Date: 2009-07-27 Publication Title: Estrogen receptor alpha controls a gene network in luminal-like breast cancer cells comprising multiple transcription factors and microRNAs. Publication Author List: Cicatiello L, Mutarelli M, Grober OM, Paris O, Ferraro L, Ravo M, Tarallo R, Luo S, Schroth GP, Seifert M, Zinser C, Chiusano ML, Traini A, De Bortoli M, Weisz A. Person Roles: submitter Person Last Name: Mutarelli Person First Name: Margherita Person Mid Initials: Person Email: [e-mail] Person Phone: Person Address: Person Affiliation: Person Roles: investigator Person Last Name: Weisz Person First Name: Alessandro Person Mid Initials: Person Email: [e-mail] Person Phone: Person Address: Person Affiliation: Experimental Design: compound_treatment_design Experimental Design: in_vivo_design Experimental Design: binding_site_identification_design Experimental Design: high_throughput_sequencing_design Experimental Design: ChIP-seq Experimental Factor Name:COMPOUND Experimental Factor Name:TIME Experimental Factor Name:IMMUNOPRECIPITATE Experimental Factor Type: compound Experimental Factor Type: time Experimental Factor Type: immunoprecipitate Quality Control Type: real_time_PCR_quality_control Quality Control Type: technical_replicate